Product Highlight

• The serum-free medium of mesenchymal stem cells specially designed for adipose-derived cells can harvest 4.5x10⁶ primary cells from 10 mL of adipose tissue and can be continuously sub-cultured to P20 without changing the medium.
• A truly serum-free pure factor system, with no human-derived or animal-derived components, for which biocompatibility and toxicity experiments have been specifically conducted to guarantee the safety of the culture medium, and with more focuses on safety.
• Suitable for declaration of stem cell drugs: the product performance is witnessed during business cooperation with dozens of clinical hospitals and pharmaceutical companies.
• It is optimized for mesenchymal stem cell populations, which are free of animal and human derived components.
• This product is specifically developed for adipose-derived mesenchymal stem cells.
• Without human or animal-derived components, more suitable for declaration and clinical trial research of stem cell drugs.
• US FDA Class II Medical Device Registration No. 510(K): K190983
• Tested for biocompatibility and toxicity, showing excellent and more safety-focused performance

Our Advantages

This product is specifically developed for adipose-derived mesenchymal stem cells, as a true serum-free pure factor culture system, without human or animal-derived components, more suitable for declaration and clinical trial research of stem cell drugs. This product has excellent performance, enabling 10 mL of adipose tissue to obtain 4.5e6 primary adipose stem cells, and the primary cells can be harvested in 6-8 days. It supports continuous passages to P20 without medium replacement and keeps the surface characteristics of mesenchymal stem cell stable.

Product Specifications:

Osmotic pressure	280-350mOsm/kg
Endotoxin	<0.25EU/mL
Sterility	Bacteria and fungi shall not be detected
Mycoplasma	shall not be detected
pH	6.8-7.8
Appearance	Light yellow - yellow clear and transparent liquid
Volume	500mL/bottle

ADSC culture

The medium can be used for separating primary ADSCs and subculturing ADSCs stably up to 20 passages.

Data of Continuous Subculturing

Passage	Inoculum density (cells/cm2)	Culture duration	Confluence	Cells harvested from 150 dishes	Amplification factor	Total harvested cells	Total amplification factor
Primary		12-14 days		4.50E+06		4.50E+06
P1	8000	72hrs	80-90%	2.80E+06	14.02	6.31E+07	15.45
P2		72hrs		3.47E+06	17.33	1.09E+09	268
P3		72hrs		2.83E+06	14.15	1.55E+10	3789
P4		72hrs		3.10E+06	15.51	2.40E+11	5.88E+04
P5		72hrs		2.65E+06	13.24	3.18E+12	7.78E+05
P6		72hrs		2.58E+06	12.89	4.10E+13	1.00E+07
P7		72hrs		2.63E+06	13.17	5.39E+14	1.32E+08
P8		72hrs		2.40E+06	12.02	6.48E+15	1.59E+09
P9		72hrs		2.51E+06	12.55	8.14E+16	1.99E+10
P10		72hrs		1.89E+06	9.45	7.69E+17	1.88E+11
P11		72hrs		1.98E+06	9.9	7.61E+18	1.86E+12
P12		72hrs		1.56E+06	7.82	5.95E+19	1.46E+13
P13		72hrs		1.53E+06	7.66	4.56E+20	1.12E+14
P14		72hrs		1.40E+06	7	3.19E+21	7.82E+14
P15		72hrs		1.47E+06	7.33	2.34E+22	5.73E+15
P16		72hrs		1.49E+06	7.43	1.74E+23	4.26E+16
P17		72hrs		1.54E+06	7.68	1.33E+24	3.27E+17
P18		72hrs		1.41E+06	7.03	9.38E+24	2.30E+18
P19		72hrs		1.47E+06	7.37	6.92E+25	1.69E+19
P20		96hrs		1.46E+06	7.29	5.04E+26	1.23E+20
*The above data was obtained from serum-free medium and stem cell mild digestive enzymes by Yocon Biology primary separation technique. Different umbilical cord samples and different separation techniques may lead to significant differences in results.

Cell Morphology